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goat polyclonal anti human tlr3  (R&D Systems)


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    R&D Systems goat polyclonal anti human tlr3
    (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for <t>TLR3,</t> TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).
    Goat Polyclonal Anti Human Tlr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti human tlr3/product/R&D Systems
    Average 94 stars, based on 21 article reviews
    goat polyclonal anti human tlr3 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5"

    Article Title: Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0160875

    (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for TLR3, TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).
    Figure Legend Snippet: (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for TLR3, TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).

    Techniques Used: Control, Western Blot, Translocation Assay, Immunofluorescence, Staining



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    goat polyclonal anti human tlr3  (R&D Systems)
    94
    R&D Systems goat polyclonal anti human tlr3
    (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for <t>TLR3,</t> TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).
    Goat Polyclonal Anti Human Tlr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat polyclonal anti human tlr3/product/R&D Systems
    Average 94 stars, based on 1 article reviews
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    goat anti human tlr3 polyclonal antibody  (R&D Systems)
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    (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for <t>TLR3,</t> TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).
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    goat anti-human tlr3 polyclonal antibodies  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology goat anti-human tlr3 polyclonal antibodies
    Immunolocalization of Toll-like receptors (TLRs) in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. TLRs were immunolocalized in NS, VV, and MC skin samples using specific anti-human TLR2, 3, 4, 7, and 9 <t>polyclonal</t> antibodies. Original magnification, ×100.
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    polyclonal goat anti human toll like receptor tlr 3 antibody  (R&D Systems)
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    R&D Systems polyclonal goat anti human toll like receptor tlr 3 antibody
    Immunolocalization of Toll-like receptors (TLRs) in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. TLRs were immunolocalized in NS, VV, and MC skin samples using specific anti-human TLR2, 3, 4, 7, and 9 <t>polyclonal</t> antibodies. Original magnification, ×100.
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    Image Search Results


    (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for TLR3, TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).

    Journal: PLoS ONE

    Article Title: Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5

    doi: 10.1371/journal.pone.0160875

    Figure Lengend Snippet: (A) HLECs were stimulated with Poly(I:C) at 0.1, 1 and 10 μg/mL for 3, 6, 12 and 24 h. Whole cell lysates were analyzed for TLR3, TRIF, phosphorylated-IRF3, total IRF3, IκBα, phosphorylated-NF-κB, NF-κB, RIG-I, MDA5, and β-tubulin (as a loading control) by Western blot. Arrowheads indicate TLR3 (116 kDa, lower band) and TRIF (76 kDa, top band). #, indicates a nonspecific band. Immunoblots are representative of three separate experiments that all showed similar results. (B) Poly(I:C) induces nuclear translocation of NF-κB. HLEC were stimulated with medium (NT) or 5 μg/mL Poly(I:C) for 24 h. Immunofluorescence staining was performed using antibodies against NF-κB (red) and nuclei were counterstained with Hoechst 33342 (blue). Images are representative of three separate experiments (400x total magnification).

    Article Snippet: Goat polyclonal anti-human TLR3 (catalog #AF1487) was purchased from R&D Systems (Minneapolis, MN).

    Techniques: Control, Western Blot, Translocation Assay, Immunofluorescence, Staining

    Immunolocalization of Toll-like receptors (TLRs) in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. TLRs were immunolocalized in NS, VV, and MC skin samples using specific anti-human TLR2, 3, 4, 7, and 9 polyclonal antibodies. Original magnification, ×100.

    Journal: Journal of Korean Medical Science

    Article Title: Expression of Toll-Like Receptors in Verruca and Molluscum Contagiosum

    doi: 10.3346/jkms.2008.23.2.307

    Figure Lengend Snippet: Immunolocalization of Toll-like receptors (TLRs) in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. TLRs were immunolocalized in NS, VV, and MC skin samples using specific anti-human TLR2, 3, 4, 7, and 9 polyclonal antibodies. Original magnification, ×100.

    Article Snippet: They were incubated overnight at 4°C with goat anti-human TLR3 polyclonal antibodies (1:50 in PBS, Santa Cruz Biotechnology).

    Techniques:

    Immunolocalization of IFN-β and TNF-α in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. IFN-β (A) and TNF-α (B) were immunolocalized in NS, VV, and MC skin samples using specific anti-human IFN-β and TNF-α polyclonal antibodies. IFN-β (red) and TLR3 (green) co-localization studies in human NS, VV, and MC skin samples were examined in C . Original magnification, ×100.

    Journal: Journal of Korean Medical Science

    Article Title: Expression of Toll-Like Receptors in Verruca and Molluscum Contagiosum

    doi: 10.3346/jkms.2008.23.2.307

    Figure Lengend Snippet: Immunolocalization of IFN-β and TNF-α in normal skin (NS), verruca vulgaris (VV), and molluscum contagiosum (MC) skin lesions. IFN-β (A) and TNF-α (B) were immunolocalized in NS, VV, and MC skin samples using specific anti-human IFN-β and TNF-α polyclonal antibodies. IFN-β (red) and TLR3 (green) co-localization studies in human NS, VV, and MC skin samples were examined in C . Original magnification, ×100.

    Article Snippet: They were incubated overnight at 4°C with goat anti-human TLR3 polyclonal antibodies (1:50 in PBS, Santa Cruz Biotechnology).

    Techniques: